Protein_Domain
Part:BBa_J70593
Designed by: Joseph Lynch Group: Knight Lab (2010-06-18)
RFC12 ATG Head Domain
A RFC12 compatible part that codes for a single amino acid, methionine. This is a head domain meant to be assembled directly in front of internal domains.
Please remember that modification of the first few codons can drastically affect translation efficiency. If you are worried about modification of the N-terminal, design your own head domain with the first two or three codons included.
This part will leave the RFC12 scar of GCTAGT [Ala-Ser] when assembled with a downstream protein. So the first three translated amino acids will be Met-Ala-Ser.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |